In this section you will find a list of selected research papers pertaining to StaphOff's patented technologies. For additional information or discussion, please email



1. Brackman G, Breyne K, De Rycke R, Vermote A, Van Nieuwerburgh F, Meyer E, Van Calenbergh S, Coenye T. The Quorum Sensing Inhibitor Hamamelitannin Increases Antibiotic Susceptibility of Staphylococcus aureus Biofilms by Affecting Peptidoglycan Biosynthesis and eDNA Release. Sci Rep. 2016 6:20321.

2. Cobrado L, Silva-Dias A, Azevedo MM, Pina-Vaz C, Rodrigues AG. In vivo antibiofilm effect of cerium, chitosan and hamamelitannin against usual agents of catheter-related bloodstream infections. J Antimicrob Chemother. 2013 68:126-30.

3. Cobrado L, Azevedo MM, Silva-Dias A, Ramos JP, Pina-Vaz C, Rodrigues AG. Cerium, chitosan and hamamelitannin as novel biofilm inhibitors? J Antimicrob Chemother. 2012 67:1159-62.

4. Brackman G, Cos P, Maes L, Nelis HJ, Coenye T. Quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo. Antimicrob Agents Chemother. 2011 55:2655-61.

5. Madanahally KD, Adikesavan NV, Cirioni O, Giacometti A, Silvestri C, Scalise G, Ghiselli R, Sava V, Orlando F, Shoham M, Balaban N. Discovery of a quorum sensing inhibitor of drug resistant staphylococcal infections by structure-based virtual screening. Mol Pharmacol 2008; 73:1578-86.


6. Zhou Y, Zhao R, Ma B, Gao H, Xue X, Qu D, Li M, Meng J, Luo X, Hou Z. Oligomerization of RNAIII-Inhibiting Peptide Inhibits Adherence and Biofilm Formation of Methicillin-Resistant Staphylococcus aureus In Vitro and In Vivo. Microb Drug Resist. 2016; 22:193-201.

7. Ma B, Zhou Y, Li M, Yu Q, Xue X, Li Z, Da F, Hou Z, Luo X. RIP-V improves murine survival in a sepsis model by down-regulating RNAIII expression and α-hemolysin release of methicillin-resistant Staphylococcus aureus. Pharmazie. 2015 70:81-7.

8. Garrigós C, Murillo O, Lora-Tamayo J, Vivas M, Tubau F, Cabellos C, Cabo J, Ariza J. RNAIII inhibiting peptide against foreign-body infection by methicillin-resistant Staphylococcus aureus. J Infect. 2012; 65:586-8.

9. Simonetti O, Cirioni O, Cacciatore I, Baldassarre L, Orlando F, Pierpaoli E, Lucarini G, Orsetti E, Provinciali M, Fornasari E, Di Stefano A, Giacometti A, Offidani A. Efficacy of the Quorum Sensing Inhibitor FS10 Alone and in Combination with Tigecycline in an Animal Model of Staphylococcal Infected Wound. PLoS One. 2016 11:e0151956. doi: 10.1371/journal.pone.0151956.

10. Schierle CF, De la Garza M, Mustoe TA, Galiano RD. Staphylococcal biofilms impair wound healing by delaying reepithelialization in a murine cutaneous wound model. Wound Repair Regen. 2009; 17:354-9.

11.  Kiran MD, Giacometti A, Cirioni O, Balaban N. Suppression of biofilm related, device-associated infections by staphylococcal quorum sensing inhibitors. In J Artif Organs2008; 31:761-70. Review.

12.  Simonetti O, Cirioni O, Ghiselli R, Goteri G, Scalise A, Orlando F, Silvestri C, Riva A, Saba V, Madanahally KD, Offidani A, Balaban N, Scalise G, Giacometti A. RNAIII-inhibiting peptide enhances healing of wounds infected with methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2008; 52:2205-11.

13.  Cirioni O, Ghiselli R, Minardi D, Orlando F, Mocchegiani F, Silvestri C, Muzzonigro G, Saba V, Scalise G, Balaban N, Giacometti A. RNAIII-inhibiting peptide affects biofilm formation in a rat model of staphylococcal ureteral stent infection. Antimicrob Agents Chemother 2007; 51;4518-20.

14.  Balaban N, Cirioni O, Giacometti A, Ghiselli R, Braunstein JB, Silvestri C, Mocchegiani F, Saba V, Scalise G. Treatment of Staphylococcus aureus biofilm åinfection by the quorum-sensing inhibitor RIP. Antimicrob Agents Chemother 2007; 51: 2226-9.

15.  Anguita-Alonso P, Giacometti A, Cirioni O, Ghiselli R, Orlando F, Saba V, Scalise G, Sevo M, Tuzova M, Patel R, Balaban N. RNAIII-inhibiting-peptide-loaded polymethylmethacrylate prevents in vivo Staphylococcus aureus biofilm formation. Antimicrob Agents Chemother 2007; 51: 2594-6.

16.  Ghiselli R, Giacometti A, Cirioni O, Dell'Acqua G, Bergnach C, Orlando F, Mocchegiani F, Silvestri C, Skerlavaj B, Licci A, Balaban N, Zanetti M, Scalise G, Saba V.  RNAIII-inhibiting peptide in combination with the cathelicidin BMAP-28 reduces lethality in mouse models of staphylococcal sepsis. Shock 2006; 26:296-301.

17.  Cirioni O, Giacometti A, Ghiselli R, Dell'Acqua G, Orlando F, Mocchegiani F, Silvestri C, Licci A, Saba V, Scalise G, Balaban N.  RNAIII-inhibiting peptide significantly reduces bacterial load and enhances the effect of antibiotics in the treatment of central venous catheter-associated Staphylococcus aureus infections. J Infect Dis 2006; 193:180-6.

18.  Balaban N, Stoodley P, Fux CA, Wilson S, Costerton JW, Dell'Acqua G.  Prevention of staphylococcal biofilm-associated infections by the quorum sensing inhibitor RIP. Clin Orthop Relat Res 2005;  437: 48-54.

19.  Giacometti A, Cirioni O, Ghiselli R, Dell'Acqua G, Orlando F, D'Amato G, Mocchegiani F, Silvestri C, Del Prete MS, Rocchi M, Balaban N, Saba V, Scalise G. RNAIII-inhibiting peptide improves efficacy of clinically used antibiotics in a murine model of staphylococcal sepsis. Peptides 2005; 26:169-75.

20.  Ghiselli R, Giacometti A, Cirioni O, Dell'Acqua G, Mocchegiani F, Orlando F, D'Amato G, Rocchi M, Scalise G, Saba V. RNAIII-inhibiting peptide and/or nisin inhibit experimental vascular graft infection with methicillin-susceptible and methicillin-resistant Staphylococcus epidermidis. Eur J Vasc Endovasc Surg 2004; 27:603-7. 

21.  Domenico P, Gurzenda E, Giacometti A, Cirioni O, Ghiselli R, Orlando F, Korem M, Saba V, Scalise G, Balaban N.  BisEDT and RIP act in synergy to prevent graft infections by resistant staphylococci. Peptides 2000; 25:2047-53.

22.  Dell'Acqua G, Giacometti A, Cirioni O, Ghiselli R, Saba V, Scalise G, Gov Y, Balaban N. Suppression of drug-resistant Staphylococcal Infections by the quorum-sensing inhibitor RNAIII-inhibiting peptide. J Infect Dis 2004; 190:318-20.

23.  Balaban N, Gov Y, Giacometti A, Cirioni O, Ghiselli R, Mocchegiani F, Orlando F, D'Amato G, Saba V, Scalise G, Bernes S, Mor A. A chimeric peptide composed of a dermaseptin derivative and an RNA III-inhibiting peptide prevents graft-associated infections by antibiotic-resistant staphylococci. Antimicrob Agents Chemother 2004;  48:2544-50.

24.  Cirioni O, Giacometti A, Ghiselli R, Dell'Acqua G, Gov Y, Kamysz W, Lukasiak J, Mocchegiani F, Orlando F, D'Amato G, Balaban N, Saba V, Scalise G. Prophylactic efficacy of topical temporin A and RNAIII-inhibiting peptide in a subcutaneous rat Pouch model of graft infection attributable to staphylococci with intermediate resistance to glycopeptides. Circulation 2003; 108:767-71.

25.  Giacometti A, Cirioni O, Gov Y, Ghiselli R, Del Prete MS, Mocchegiani F, Saba V, Orlando F, Scalise G, Balaban N, Dell'Acqua G. RNA III inhibiting peptide inhibits in vivo biofilm formation by drug-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2003; 47:1979-83.

26.  Balaban N, Giacometti A, Cirioni O, Gov Y, Ghiselli R, Mocchegiani F, Viticchi C, Del Prete MS, Saba V, Scalise G, Dell'Acqua G. Use of the quorum-sensing inhibitor RNAIII-inhibiting peptide to prevent biofilm formation in vivo by drug-resistant Staphylococcus epidermidis. J Infect Dis 2003; 187:625-30. 

27.  Balaban N, Collins LV, Cullor JS, Hume EB, Medina-Acosta E, Vieira da Motta O, O'Callaghan R, Rossitto PV, Shirtliff ME, Serafim da Silveira L, Tarkowski A, Torres JV. Prevention of diseases caused by Staphylococcus aureus using the peptide RIP. Peptides 2000; 21:1301-11.

28.  Balaban N, Goldkorn T, Nhan RT, Dang LB, Scott S, Ridgley RM, Rasooly A, Wright SC, Larrick JW, Rasooly R, Carlson JR. Autoinducer of virulence as a target for vaccine and therapy against Staphylococcus aureus. Science 1998; 280:438-40.

Clinical Cases

29.  Wolcott R, Lopez-Leban F, Kiran MD, Balaban N. Wound healing by biofilm eradication, in Biofilm Perspectives, Hans-Curt Flemming, Ed. Springer, 2011.

30.  Lopez-Leban F, Kiran MD, Wolcott R, Balaban N. Molecular mechanisms of RIP, an effective inhibitor of chronic infections. Int J Artif Organs. 2010; 33:582-9.

31.  Wolcott R. The control of Biofilm Infections by Signal Manipulation. Balaban N, Ed. Springer. 2008.

32. Wolcott RD, Rhoads, DD. A study of biofilm-based woundmanagement in subjects with critical limb ischaemia. Journal of wound care 2008; 1 7:145-155.